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2015-12-1 · Each tooth sectioned three times perpendicular to the line axis of the tooth; with a low-speed diamond saw (IsoMet, Buehler, USA) under a water coolant. The first cut created a smooth enamel surface. The second cut was about 2 mm under dentin-enamel junction and the third one was 2 mm more apically to the second cut.Get Price